技術(shù)文章/ARTICLE
小與除任我工等每利加習(xí)滿立質(zhì)約于階放(油價(jià)/推薦)
點(diǎn)擊次數(shù)：2922 發(fā)布時(shí)間：2022/9/14 21:39:30
檢測(cè)原理方正姚體試劑盒采用雙抗體夾心法酶聯(lián)免疫吸附試驗(yàn)。往預(yù)先包被人乳酸脫氫酶（方正姚體）捕獲抗體的包被微孔中，依次加入標(biāo)本、標(biāo)準(zhǔn)品、方正姚體_GBK">HRP標(biāo)記的檢測(cè)抗體，經(jīng)過溫育并徹di洗滌。用底物TMB顯色，TMB在過氧化物酶的催化下轉(zhuǎn)化成藍(lán)色，并在酸的作用下轉(zhuǎn)化成終的黃色。顏色的深淺和樣品中的人乳酸脫氫酶（LDH方正姚體_）呈正相關(guān)。用酶標(biāo)儀在方正姚體_450nm 波長(zhǎng)下測(cè)定吸方正姚體_光度（方正姚體_OD 值），計(jì)算樣品濃度。樣品收集、處理及保存方法1. 血清：使用不含熱原和內(nèi)毒素的試管，操作過程中避免任何細(xì)胞刺激，收集血液后，3000轉(zhuǎn)離心10分鐘將血清和紅細(xì)胞迅速小心地分離。2. 血漿：EDTA、檸檬酸鹽或肝素抗凝。3000轉(zhuǎn)離心30分鐘取上清。3. 細(xì)胞上清液：3000轉(zhuǎn)離心10分鐘去除顆粒和聚合物。4. 組織勻漿：將組織加入適量生理鹽水搗碎。3000轉(zhuǎn)離心10分鐘取上清。5. 保存：如果樣本收集后不及時(shí)檢測(cè)，請(qǐng)按一次用量分裝，凍存于-20℃，避免反復(fù)凍融，在室溫下解凍并確保樣品均勻地充分解凍。
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